Breeding plants with genes from one parent
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Scientists are a step closer to breeding plants with genes from only one parent. New research led by plant biologists at the University of California, Davis, published Nov. 19 in Science Advances, shows the underlying mechanism behind eliminating half the genome and could make for easier and more rapid breeding of crop plants with desirable traits such as disease resistance.
The work stems from a discovery made over a decade ago by the late Simon Chan, associate professor of plant biology in the UC Davis College of Biological Sciences, and colleagues.
Plants, like other sexual organisms, inherit a matching set of chromosomes from each parent. In order to transmit a favorable trait, such as pest or drought resistance, to all their offspring, the plant would have to carry the same genetic variant on each chromosome. But creating plants that “breed true” in this way can take generations of cross-breeding.
In 2010, Chan and postdoctoral fellow Ravi Maruthachalam serendipitously discovered a way to eliminate the genetic contribution from one parent while breeding the lab plant Arabidopsis. They had modified a protein called CENH3, found in the centromere, a structure in the center of a chromosome. When they tried to cross wildtype Arabidopsiswith plants with modified CENH3, they got plants with half the normal number of chromosomes. The part of the genome from one parent plant had been eliminated to create a haploid plant.
That work was published in Nature in March 2010, setting off efforts to achieve the same result in crop plants such as maize, wheat and tomato.
Clearing up a mystery
But replicating Chan’s exact strategy outside Arabidopsis has so far proved fruitless, said Professor Luca Comai, UC Davis Department of Plant Biology and Genome Center, who is senior author on the new paper. Recently, other labs have created plants with one set of chromosomes by manipulating CENH3, but it’s not clear how the results are related.
“The mechanistic basis of CENH3 effects on haploid induction was mysterious,” Comai said. There appear to be different rules for each species, he said.
Much of that mystery has now been cleared up. Mohan Marimuthu, researcher at the UC Davis Genome Center and Department of Plant Biology, with Comai, Maruthachalam (now at the Indian Institute of Science Education and Research, Kerala) and colleagues found that when CENH3 protein is altered, it is removed from the DNA in the egg before fertilization, weakening the centromere.
“In the subsequent embryonic divisions, the CENH3-depleted centromeres contributed by the egg fail to compete with the CENH3-rich ones contributed by the sperm and the female genome is eliminated,” Comai said.
The finding that any selective depletion of CENH3 engenders centromere weakness explains the original results by Chan and Maruthachalam as well as new results from other labs in wheat and maize, Comai said. This new knowledge should make it easier to induce haploids in crop plants, he said.
Additional authors on the paper are: at UC Davis, Anne Britt and Sundaram Kuppu; Ramesh Bondada, Indian Institute of Science Education and Research; and Ek Han Tan, University of Maine.
Mohan P. A. Marimuthu et al, Epigenetically mismatched parental centromeres trigger genome elimination in hybrids, Science Advances (2021). DOI: 10.1126/sciadv.abk1151. www.science.org/doi/10.1126/sciadv.abk1151
Citation:
Breeding plants with genes from one parent (2021, November 19)
retrieved 19 November 2021
from https://phys.org/news/2021-11-genes-parent.html
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Medical Information Today has strict sourcing guidelines as well as draws just from peer-reviewed research studies, academic study institutions, and medical journals and organizations. You can learn more about how we ensure our material is precise and existing by reading our editorial plan. Teeth can become sensitive when enamel, their safety top layer, comes to be worn down. The authors of a 2011 testimonial note that the topical application of capsaicin can help soothe some types of pain. Scientists are not certain exactly just how this works, but they think that capsaicin may numb nerve fibers, making them much less able to deliver discomfort signals. Oil drawing is a conventional Ayurvedic practice stemming from India, which involves swishing oil around the mouth for several mins prior to spewing it out.
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